Journal: Journal of Clinical Medicine

Article Title: Uterine Endometrium Microbiome in Women with Repeated Implantation Failure Complicated by Endometriosis

doi: 10.3390/jcm13164605

Figure Lengend Snippet: Chronic endometritis and uterine endometrium microbiome in repeated implantation failure women.

Article Snippet: The samples were placed in the middle part of the aspiration tube and soaked in an OMNIgene ® -VAGINAL microbiome kit (DNA Genotek Inc., Ottawa, ON, Canada).

Techniques: Clinical Proteomics, Staining

Journal: Microbiome

Article Title: Lignocellulose degradation at the holobiont level: teamwork in a keystone soil invertebrate

doi: 10.1186/s40168-018-0536-y

Figure Lengend Snippet: Model for lignocellulose degradation in the A. vulgare holobiont. Diagrams represent the CAZy families contributed by the host (red) and the microbiome (blue). (I) Lignin would be partially degraded to release cellulose and hemicellulose. (II) Cellulose would be degraded by the action of endoglucanases and β-glucosidases. A high number of β-glucosidases and mechanical fragmentation by A. vulgare could compensate for the lack of exoglucanases. (III) The A. vulgare holobiont could degrade most types of hemicellulose due to the high diversity of Debranching enzymes* (CE1, CE3, CE4, CE5, CE6, CE7, CE12, GH3, GH4, GH43, GH51), Endo-hemicellulases* (GH5, GH8, GH9, GH10, GH11, GH16, GH30, GH43, GH51, GH53, GH74, GH113, GH128, GH134), and Exo-hemicellulases* (GH1, GH2, GH3, GH4, GH5, GH27, GH29, GH30, GH31, GH35, GH36, GH39, GH42, GH43, GH51, GH57, GH116, GH120)

Article Snippet: Equimolar amounts of DNA from 7 to 10 biological replicates of the same tissue and sample type (i.e., origin, gender, Wolbachia infection status) were pooled and prokaryotic DNA was enriched in each pool using the NEBNext® Microbiome DNA Enrichment kit (New England Biolabs) according to the manufacturer’s instructions.

Techniques:

Journal: Microbiome

Article Title: Lignocellulose degradation at the holobiont level: teamwork in a keystone soil invertebrate

doi: 10.1186/s40168-018-0536-y

Figure Lengend Snippet: Lignocellulose-degrading enzymes and their associated microbial community in ( a ) isopods from the laboratory and ( b ) isopods from a natural population. c The microbial taxa contributing lignocellulose-binding modules in both field and laboratory specimens

Article Snippet: Equimolar amounts of DNA from 7 to 10 biological replicates of the same tissue and sample type (i.e., origin, gender, Wolbachia infection status) were pooled and prokaryotic DNA was enriched in each pool using the NEBNext® Microbiome DNA Enrichment kit (New England Biolabs) according to the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Frontiers in Public Health

Article Title: Student-Driven Course-Based Undergraduate Research Experience (CUREs) Projects in Identifying Vaginal Microorganism Species Communities to Promote Scientific Literacy Skills

doi: 10.3389/fpubh.2022.870301

Figure Lengend Snippet: Stage goals, activities, and focal skills associated with each phase of the project.

Article Snippet: NCBI's Sequence Read Archive (BioProject ID: PRJNA46877) was used as a control for vaginal microbiome 16S rDNA reference sequence analysis , and the generated contigs used the Lactobacillus gasseri (ATCC 9857) strain as the reference genome.

Techniques: Activity Assay, Amplification, Sequencing, Functional Assay

Journal: Frontiers in Public Health

Article Title: Student-Driven Course-Based Undergraduate Research Experience (CUREs) Projects in Identifying Vaginal Microorganism Species Communities to Promote Scientific Literacy Skills

doi: 10.3389/fpubh.2022.870301

Figure Lengend Snippet: List of main associated literature in planning phase students prepared of Vaginal Microbiome.

Article Snippet: NCBI's Sequence Read Archive (BioProject ID: PRJNA46877) was used as a control for vaginal microbiome 16S rDNA reference sequence analysis , and the generated contigs used the Lactobacillus gasseri (ATCC 9857) strain as the reference genome.

Techniques: Infection, Membrane, Amplification, Sequencing

Journal: Frontiers in Public Health

Article Title: Student-Driven Course-Based Undergraduate Research Experience (CUREs) Projects in Identifying Vaginal Microorganism Species Communities to Promote Scientific Literacy Skills

doi: 10.3389/fpubh.2022.870301

Figure Lengend Snippet: Steps of flow chart for 16S rRNA gene amplicon sequencing.

Article Snippet: NCBI's Sequence Read Archive (BioProject ID: PRJNA46877) was used as a control for vaginal microbiome 16S rDNA reference sequence analysis , and the generated contigs used the Lactobacillus gasseri (ATCC 9857) strain as the reference genome.

Techniques: Amplification, Sequencing